Composite

Part:BBa_K3581020:Design

Designed by: HUI Wan Hei   Group: iGEM20_HKUST   (2020-10-24)


T7 parts without hagfish intermediate filaments with TorA signal peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 288
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 137
    Illegal BsaI.rc site found at 269


Design Notes

The hagfish subunit is removed from this part for maximizing protein secretion even if the protein is not hagfish intermediate filaments.



Source

All parts are from iGEM registry.

References

1. England, N. (n.d.). TEV Protease | NEB. Retrieved October 13, 2020, from https://international.neb.com/products/p8112-tev-protease#Product%20Information

2. Wikipedia. (2020, October 14). TEV Protease. Wikipedia. Retrieved October 13, 2020, from https://en.wikipedia.org/wiki/TEV_protease

3. Palmer, I., & Wingfield, P. T. (2004). Preparation and extraction of insoluble (inclusion-body) proteins from Escherichia coli. Current protocols in protein science, Chapter 6, Unit–6.3. https://doi.org/10.1002/0471140864.ps0603s38

4. https://parts.igem.org/Part:BBa_I712074

5. https://parts.igem.org/Part:BBa_B0030

6. https://parts.igem.org/Part:BBa_B0016